Methods of artificial insemination. Transvaginal insemination: Fresh or chilled semen is deposited into the vagina with a pipette and syringe, attempting to deposit the semen as close to the cervix as possible. If done incorrectly, transvaginal AI can cause damage to a dog's reproductive tract, and should only be done by someone who is.. Fresh-chilled. Fresh-chilled semen involves the dilution of ejaculated semen in special canine extenders which contain egg yolk and buffers that protect the sperm during the cooling process. The extended semen is then slowly cooled to 4˚C and can remain viable at 4˚C for up to 3-4 days. (Semen from some stud dogs has been reported to maintain.

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Although there is a mediocre price variation, the average prices of a canine reproduction clinic are listed below. Progesterone Test - £35. Artificial Insemination - £40. Ultrasound Scan - £30. Sperm Analysis - £40. Stud Dogs - £500+. Advice - £0. Remember - the above prices are a guide only.. The amount of semen that is required to give the best chance of a successful insemination varies depending on the size of the female. For bitches less than 10 pounds (5 kg) 1.5 to 3 mls of semen are required per insemination. For bitches 10 to 50 pounds (5 to 25 kg), 3 to 5 mls of semen is necessary. For bitches over 50 pounds (25 kg), 5 to 8.